Acta Biochimica Polonica, Paper in Press, No. 1668, Regular paper

Specific binding sites for progesterone and 17β-estradiol in cells of Triticum aestivum L.

Anna Janeczko1, e-mail, Bogusława Budziszewska2, Andrzej Skoczowski1 and Małgorzata Dybała3

1Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland; 2Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland; 3Laboratory of Pharmacobiology, Department of Cytobiology and Histochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland

Keywords: Triticum aestivum L., progesterone, 17β-estradiol, radioligand binding assay, vernalization

The presence and location of specific binding sites for progesterone and 17β-estradiol in cells of wheat were estimated using radioligand binding assay. Membrane and cytosolic fractions of non-vernalized and vernalized plants were tested using tritium-labelled ligands. Specific binding of [3H]progesterone and [3H]17β-estradiol occurs in wheat cells. The binding sites are located in membranes and in the cytosol. Specific binding of [3H]17β-estradiol is higher in the membranes than in the cytosol. Specific binding of both ligands in the cytosolic fraction is higher in vernalized plants than in non-vernalized ones. The possibility of the occurrence of steroid binding proteins specific for progesterone and 17β-estradiol, putative steroid receptors for these steroids in Triticum aestivum L., is discussed.

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