The Journal of the Polish Biochemical Society
and of the Committee of Biochemistry and Biophysics
Polish Academy of Sciences

Acta Biochimica Polonica
Vol. 63 No. 2/2016

Acta Biochimica Polonica
Supplement 1, 2015
Frontiers in Cancer Prevention

Acta Biochimica Polonica
Supplement 2, 2015
Weigl Conference on Microbiology

Selected Review from the Current Issue:

The phenomenon of fluorescence in immunosensors

Aleksandra Kłos-Witkowska

The phenomenon of fluorescence in immunosensors is described in this paper. Both structure and characteristics of biosensors and immunosensors are presented. Types of immunosensors and the response of bioreceptor layers to the reaction with analytes as well as measurements of electrochemical, piezoelectric and optical parameters in immunosensors are also presented. In addition, detection techniques used in studies of optical immunosensors based on light-matter interactions (absorbance, reflectance, dispersion, emission) such as: UV/VIS spectroscopy, reflectometric interference spectroscopy (RIfs), surface plasmon resonance (SPR), optical waveguide light-mode spectroscopy (OWLS), fluorescence spectroscopy. The phenomenon of fluorescence in immunosensors and standard configurations of immunoreactions between an antigen and an antibody (direct, competitive, sandwich, displacement) is described. Fluorescence parameters taken into account in analyses and fluorescence detection techniques used in research of immunosensors are presented. Examples of immunosensor applications are given.

Acta Biochimica Polonica, 63(2), 215-221, Review, DOI: 10.18388/abp.2015_1231

Acta Biochimica Polonica is indexed in: Current Contents, Biochem. and Biophys. Citation Index, BIOSIS, Chemical Abstracts, Excerpta Medica, Medline, Index Copernicus, CBR

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